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DNA Ladder Step50 plus

Features: DNA ladder 50 bp is ideal for estimating the molecular weight of linear, double stranded DNA fragments from 50 – 1500 bp in agarose gel.

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DNA ladder Step50 plus is suitable for using as a standard for estimating of length and quantity analysis of double stranded DNA molecules from 50 to 1500 bp in agarose gel. Prepared from a plasmid DNA, ladder contains 13 DNA fragments. For easy reference on agarose gels, the 200 and 500 bp fragments areconcentrated.
DNA ladder Step50 plus is ready-to-use. This ladder can be stained with intercalating agents as ethidium bromide or SYBR Green Dye.
 
DNA ladder Step50 plus visualized by ethidium bromide staining on a 2% TAE agarose gel:
 

DNA ladder concentration: 0,1 mg/ml.
 
Storage buffer: 10 mM Tris-HCl (pH 8.0), 5 mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylencyanol.
 
Storage conditions: -20°C / 12 months.
 
Recommendations for use:
  • DNA ladder is ready-to-use;
  • apply 3-5 μl of ready-to-use  DNA ladder Step50 plus on a 5 mm lane of the agarose gel;
  • visualize the DNA bands  by ethidium bromide or SYBR Green  staining.