БиоМастер HS-Taq ПЦР-Спец (2×)
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Особенности
Смесь оптимизирована для проведения эффективной и воспроизводимой ПЦР c “горячим” стартом на сложно-структурированных и/или GC-богатых ДНК-матрицах.
MH011-200
Количество реакций (50 мкл)
200
3 050 ₽
MH011-1020
Количество реакций (50 мкл)
1020
14 700 ₽
Описание
БиоМастер HS - Taq ПЦР-Спец (2×) предназначен для проведения ПЦР ДНК-матриц со сложной пространственной структурой или с GC-богатыми участками. Набор БиоМастер HS - Taq ПЦР-Спец (2×) содержит 2× реакционную смесь БиоМастер HS - Taq ПЦР-Спец (2×) 2 и стерильную воду. В состав БиоМастер HS - Taq ПЦР-Спец (2×) Taq ДНК-полимераза c “горячим” стартом, смесь дезоксинуклеозидтрифосфатов, 2× ПЦР-буфер, Mg2+ .Taq ДНК-полимеразы за счет повышения её стабильности во время ПЦР. БиоМастер HS - Taq ПЦР-Спец (2×) 2 позволяет легко оптимизировать реакционную смесь под конкретную систему матрица-праймеры. Представленная форма набора для проведения ПЦР экономит время и снижает вероятность контаминации за счет малого числа шагов пипетирования.
Области применения
Наработка ПЦР-продуктов для ТА-клонирования ПЦР классическая с "горячим" стартом ПЦР сложных и GC-богатых участков ПЦР с обратной транскрицией одно- и двухшаговая Свойства реакционной смеси
Смесь оптимизирована для специфичной работы HS-Taq ДНК-полимеразы, длительного хранения (БиоМастер HS - Taq ПЦР-Спец (2×)
Преимущества использования
Амплифицирует матрицы с GC-составом более 66%
Фермент с “горячим” стартом повышает специфичность, чувствительность и выход реакции;
Для активации HS-Taq ДНК-полимеразы требуется не более 5 мин;
Сокращается время на подготовку реакции;
Снижается вероятность контаминации при смешивании компонентов ПЦР;
Стандартизируются условия постановки однотипных реакций (снижается погрешность при смешивании компонентов ПЦР в разных экспериментах);
Возможность ТА клонирования продуктов ПЦР за счет выступающих на концах амплифицированных фрагментов ДНК дезоксиаденозиновых остатков.
Хранение
в месте, защищенном от попадания света: при +25 °С – 7дней; при +4 °С – 6 месяцев; при -20°С – 1 год; не более 50 циклов замораживания-размораживания.
Транспортировка
при 0 - +4 °С, допускается транспортировка при комнатной температуре до 3-х дней.
Паспорт на продукцию
Мы тщательно готовим нашу продукцию и готовы подтвердить ее пригодность паспортом.
На каждой коробке нашей продукции есть уникальный номер-лот в формате АА-000-0000.
