We are constantly improving the protocol for the kit. Please use the protocol provided with the product.
Updated March 2022
The kit is designed for DNA/RNA isolation and purification from animal and bacterial cells cultures, swabs, viruses. Lysis buffer lyses cell membrane releasing nucleic acids. Subsequent steps are precipitation and washing of nucleic acids (DNA/RNA) followed by dissolving of samples.
Note: Lysis buffer contains nucleic acids co-precipitant, therefore using additional co-precipitant reagent using isn’t necessary.
Important! RNase or DNase treatment is required if clean DNA or RNA is needed.
The isolated DNA can be used for PCR, nick-translation, sequencing and other genetic engineering applications.
The isolated RNA can be used for RT-PCR, sequencing and other genetic engineering applications.
CAUTION! When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. You must follow the rules of general and personal safety when working with the kit. Lysis buffer LB contains guanidine thiocyanate solution, which is irritating and toxic. Buffer are toxic in contact with skin and insides, causing burns. In case of a contact with skin, wash immediately with plenty of water and detergents. Visit a doctor if necessary.
CAUTION! Precipitation buffer PB isopropanol, which is irritating and toxic. Don’t work with this buffer near the open fire.