We are constantly improving the protocol for the kit. Please use the protocol provided with the product.
Updated January 2022
The kit is designed for isolation and purification of DNA (from 50 to 10000 base pairs) from reaction mixtures. For instance, it is possible to purify DNA from dNTP, enzymes, unbound low molecular weight radioactive and fluorescent labels, etc. The method of DNA isolation is based on the selective binding of nucleic acids from a lysed sample on a silica-gel membrane, followed by washing and the elution of the purified sample. It is possible to isolate up to 10-20 μg of DNA.
The isolated DNA can be used for PCR, transcription, nick-translation, sequencing, and other genetic engineering applications.
The kit does not contain phenol and chaotropic salts like guanidine thiocyanate.
CAUTION! When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. You must follow the rules of general and personal safety when working with the kit.