We are constantly improving the protocol for the kit. Please use the protocol provided with the product.
Updated March 2022
The kit is designed for isolation and purification of genomic DNA from animal cell cultures, gram-negative and gram-positive bacteria, animal and plant tissues, blood. The method of DNA isolation is based on the selective binding of nucleic acids from a lysed sample on a silica-gel membrane, followed by washing and the elution of the purified sample. It is possible to isolate up to 30-50 μg of DNA.
The isolated DNA can be used for PCR, nick-translation, and other genetic engineering applications.
CAUTION! When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. You must follow the rules of general and personal safety when working with the kit. Lysis LB and wash WB1 buffers contain guanidine thiocyanate solution, which is irritating and toxic. Buffers are toxic in contact with skin and insides, causing burns.
In case of a contact with skin, wash immediately with plenty of water and detergents. Visit a doctor if necessary.