RNA isolation kit for isolation of RNA from swabs, viruses

Features
Kit components

NAmagp100

100 preps

Lysis buffer LB

45 ml

Wash buffer WB

(concentrate)

22 ml

Elution buffer EB

10 ml

Magnetic particles

(suspension)

1.1 ml


Basic description

Important!

We are constantly improving the protocol for the kit. Please use the protocol provided with the product.

Updated January 2022

Description

The kit is designed for isolation and purification of RNA from swabs, viruses. The method of RNA isolation is based on the selective binding of nucleic acids from a lysed sample on a magnetic particles formed with iron oxide and silica, followed by washing and the elution of the purified RNA. The integrity of RNA is preserved in the process of isolation.

It is possible to extract RNA both manually by using a magnetic stand and automatically by using the automatic nucleic acid purification system Auto-Pure96 (Allsheng) and KingFisherFlex (ThermoScientific).

Important! Isolated RNA contains admixture DNA. DNAse treatment is required when using RNA in applications sensitive to the presence of DNA, for example, PCR.

RNA isolation time by the automatic stations Auto-Pure96 (Allsheng) and KingFisherFlex (ThermoScientific) is equal to 20 minutes.

Safety Information

CAUTION! When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. You must follow the rules of general and personal safety when working with the kit. Lysis LB buffer contains guanidine thiocyanate solution, which is irritating and toxic. Buffer are toxic in contact with skin and insides, causing burns.

In case of a contact with skin, wash immediately with plenty of water and detergents. Visit a doctor if necessary.

Areas of use

  • Isolation of RNA, DNA and Proteins

Benefits of using

Storage

All components of the kit, except magnetic particles, can be stored at room temperature (15-25 °C) for up to 12 months. Magnetic particles are stored at 2-8 °C for up to 12 months.

Transportation

All components of the kit including magnetic particles are shipped at room temperature.

Product passport

We carefully prepare our products and are ready to confirm their suitability with a passport.

Each box of our products has a unique lot number in the AA-000-0000 format.
Enter it in the box below and download the certificate in PDF format.