We are constantly improving the protocol for the kit. Please use the protocol provided with the product.
Updated March 2022
The kit is designed for total RNA isolation and purification from animal and bacterial cells cultures, swabs, viruses. The method of RNA isolation is based on the selective binding of nucleic acids from a lysed sample on a silica-gel membrane, followed by washing and the elution of the purified RNA. The integrity of RNA is preserved in the process of isolation. It is possible to isolate more than 50 μg of RNA per one column.
Important! Isolated RNA contains admixture DNA. DNAse treatment is required when using RNA in applications sensitive to the presence of DNA, for example, PCR.
RNAs isolated from bacterial cells (lane 1, 1 mg of E. coli) and from human cells (lane 2, 1*106 cells). About 500 ng of NAs per lane was loaded.
CAUTION! When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. You must follow the rules of general and personal safety when working with the kit. Lysis LB and wash WB1 buffers contain guanidine thiocyanate solution, which is irritating and toxic. Buffers are toxic in contact with skin and insides, causing burns.
In case of a contact with skin, wash immediately with plenty of water and detergents. Visit a doctor if necessary.