RNA isolation kit for isolation of total RNA from animal and bacterial cells, swabs, viruses on columns

Features

Contents

RU-50

50 preps

RU-250

250 preps

Lysis buffer LB

25 ml

2x60 ml

Wash buffer WB1

(concentrate)

9 ml

2x21 ml

Wash buffer WB2

(concentrate)

6 ml

2x14 ml

Elution buffer EB

2x5 ml

50 ml

Collection tubes and spin columns

50 pcs

250 pcs

Application

Equipment and Reagents to be Supplied by User

Microcentrifuge with rotor for 1.5 ml tubes, speed 10000 rcf

1.5 ml microcentrifuge tubes

Ethanol, 96-100%

14.3 M 2-mercaptoethanol (2-ME) (commercially available solutions are usually 14.3 M) or, alternatively, 2 M dithiothreitol (DTT) water solution

Basic description

Important!

We are constantly improving the protocol for the kit. Please use the protocol provided with the product.

Updated March 2022

Description

The kit is designed for total RNA isolation and purification from animal and bacterial cells cultures, swabs, viruses. The method of RNA isolation is based on the selective binding of nucleic acids from a lysed sample on a silica-gel membrane, followed by washing and the elution of the purified RNA. The integrity of RNA is preserved in the process of isolation. It is possible to isolate more than 50 μg of RNA per one column.

Important! Isolated RNA contains admixture DNA. DNAse treatment is required when using RNA in applications sensitive to the presence of DNA, for example, PCR.


RNAs isolated from bacterial cells (lane 1, 1 mg of E. coli) and from human cells (lane 2, 1*106 cells). About 500 ng of NAs per lane was loaded.


Safety Information

CAUTION! When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. You must follow the rules of general and personal safety when working with the kit. Lysis LB and wash WB1 buffers contain guanidine thiocyanate solution, which is irritating and toxic. Buffers are toxic in contact with skin and insides, causing burns.

In case of a contact with skin, wash immediately with plenty of water and detergents. Visit a doctor if necessary.

Areas of use

  • Isolation of RNA, DNA and Proteins

Benefits of using

Storage

All components of the kit can be stored at room temperature (15-25 °C) for up to 12 months.

Transportation

All components of the kit are shipped at room temperature.

Product passport

We carefully prepare our products and are ready to confirm their suitability with a passport.

Each box of our products has a unique lot number in the AA-000-0000 format.
Enter it in the box below and download the certificate in PDF format.