Array
(
[TEMPLATE_THEME] => blue
[SHOW_MAX_QUANTITY] => N
[SHOW_OLD_PRICE] => N
[SHOW_CLOSE_POPUP] => N
[SHOW_DISCOUNT_PERCENT] => N
[DISCOUNT_PERCENT_POSITION] => bottom-right
[LABEL_PROP] => Array
(
)
[LABEL_PROP_MOBILE] => Array
(
)
[LABEL_PROP_POSITION] => top-left
[PRODUCT_SUBSCRIPTION] => N
[MESS_BTN_BUY] => Купить
[MESS_BTN_ADD_TO_BASKET] => В корзину
[MESS_BTN_SUBSCRIBE] => Подписаться
[MESS_BTN_DETAIL] => Подробнее
[MESS_NOT_AVAILABLE] => Нет в наличии
[MESS_BTN_COMPARE] => Сравнение
[SHOW_SLIDER] => N
[SLIDER_INTERVAL] => 5000
[SLIDER_PROGRESS] => N
[USE_ENHANCED_ECOMMERCE] => N
[DATA_LAYER_NAME] =>
[BRAND_PROPERTY] =>
[DISPLAY_NAME] => Y
[USE_RATIO_IN_RANGES] => Y
[IMAGE_RESOLUTION] => 16by9
[DETAIL_PICTURE_MODE] => Array
(
)
[ADD_DETAIL_TO_SLIDER] => N
[DISPLAY_PREVIEW_TEXT_MODE] => E
[ADD_TO_BASKET_ACTION] => Array
(
[0] => BUY
)
[ADD_TO_BASKET_ACTION_PRIMARY] => Array
(
[0] => BUY
)
[USE_VOTE_RATING] => N
[VOTE_DISPLAY_AS_RATING] => rating
[USE_COMMENTS] => N
[BLOG_USE] => N
[BLOG_URL] =>
[BLOG_EMAIL_NOTIFY] =>
[VK_USE] => N
[VK_API_ID] => API_ID
[FB_USE] => N
[FB_APP_ID] =>
[BRAND_USE] => N
[BRAND_PROP_CODE] => Array
(
)
[IBLOCK_ID] => 10
[IBLOCK_TYPE] => catalog
[PROPERTY_CODE] => Array
(
)
[META_KEYWORDS] => -
[META_DESCRIPTION] => -
[BROWSER_TITLE] => NAME
[SET_CANONICAL_URL] => N
[BASKET_URL] => /en/cart/
[ACTION_VARIABLE] => action
[PRODUCT_ID_VARIABLE] => id
[SECTION_ID_VARIABLE] => SECTION_ID
[CHECK_SECTION_ID_VARIABLE] => N
[PRODUCT_QUANTITY_VARIABLE] => quantity
[PRODUCT_PROPS_VARIABLE] => prop
[CACHE_TYPE] => A
[CACHE_TIME] => 36000000
[CACHE_GROUPS] => Y
[SET_TITLE] => 1
[SET_LAST_MODIFIED] =>
[MESSAGE_404] =>
[SET_STATUS_404] => N
[SHOW_404] => N
[FILE_404] =>
[PRICE_CODE] => Array
(
[0] => base_price
)
[USE_PRICE_COUNT] =>
[SHOW_PRICE_COUNT] => 1
[PRICE_VAT_INCLUDE] =>
[PRICE_VAT_SHOW_VALUE] =>
[USE_PRODUCT_QUANTITY] => 1
[PRODUCT_PROPERTIES] => Array
(
)
[ADD_PROPERTIES_TO_BASKET] => Y
[PARTIAL_PRODUCT_PROPERTIES] => N
[LINK_IBLOCK_TYPE] =>
[LINK_IBLOCK_ID] => 0
[LINK_PROPERTY_SID] =>
[LINK_ELEMENTS_URL] => link.php?PARENT_ELEMENT_ID=#ELEMENT_ID#
[OFFERS_CART_PROPERTIES] => Array
(
)
[OFFERS_FIELD_CODE] => Array
(
)
[OFFERS_PROPERTY_CODE] => Array
(
[0] => PREVIEW_PICTURE
[1] => DETAIL_PICTURE
)
[OFFERS_SORT_FIELD] => sort
[OFFERS_SORT_ORDER] => asc
[OFFERS_SORT_FIELD2] => id
[OFFERS_SORT_ORDER2] => desc
[ELEMENT_ID] => 922
[ELEMENT_CODE] => biomaster-qrt-pcr-2-
[SECTION_ID] => 0
[SECTION_CODE] => nabory-i-smesi-ot-otpcr
[SECTION_URL] => /en/catalog/#SECTION_CODE_PATH#/
[DETAIL_URL] => /en/catalog/#SECTION_CODE_PATH#/#ELEMENT_CODE#/
[CONVERT_CURRENCY] => N
[CURRENCY_ID] =>
[HIDE_NOT_AVAILABLE] => N
[HIDE_NOT_AVAILABLE_OFFERS] => N
[USE_ELEMENT_COUNTER] => Y
[SHOW_DEACTIVATED] => N
[USE_MAIN_ELEMENT_SECTION] =>
[STRICT_SECTION_CHECK] =>
[ADD_PICT_PROP] =>
[OFFER_ADD_PICT_PROP] =>
[OFFER_TREE_PROPS] => Array
(
)
[MESS_SHOW_MAX_QUANTITY] => Stock
[RELATIVE_QUANTITY_FACTOR] => 5
[MESS_RELATIVE_QUANTITY_MANY] => in stock
[MESS_RELATIVE_QUANTITY_FEW] => only a few left
[MESS_PRICE_RANGES_TITLE] => Цены
[MESS_DESCRIPTION_TAB] => Описание
[MESS_PROPERTIES_TAB] => Характеристики
[MESS_COMMENTS_TAB] => Комментарии
[MAIN_BLOCK_PROPERTY_CODE] => Array
(
[ITEM_ID] => 0
[ITEM_PHOTOS] => 1
[ITEM_PASSPORT] => 2
[ITEM_AD_BUKLET] => 3
[ITEM_DOCUMENTATION] => 4
[ITEM_SPECIALITY] => 5
[ITEM_REACTION_SMES] => 6
)
[MAIN_BLOCK_OFFERS_PROPERTY_CODE] => Array
(
[QUANTITY_ML] => 0
[QUANTITY_REAKCIY_25] => 1
[QUANTITY_REAKCIY_50] => 2
[QUANTITY_REAKCIY_EA] => 3
[QUANTITY_MKG] => 4
[REACTION] => 5
[QUANTITY_PCS] => 6
)
[PRODUCT_INFO_BLOCK_ORDER] => Array
(
[0] => sku
[1] => props
)
[PRODUCT_PAY_BLOCK_ORDER] => Array
(
[0] => rating
[1] => price
[2] => priceRanges
[3] => quantityLimit
[4] => quantity
[5] => buttons
)
[ADD_SECTIONS_CHAIN] => 1
[ADD_ELEMENT_CHAIN] => 1
[DISPLAY_COMPARE] =>
[COMPARE_PATH] => /en/catalog/compare.php?action=#ACTION_CODE#
[USE_COMPARE_LIST] => Y
[BACKGROUND_IMAGE] =>
[COMPATIBLE_MODE] => Y
[DISABLE_INIT_JS_IN_COMPONENT] => N
[SET_VIEWED_IN_COMPONENT] => N
[USE_GIFTS_DETAIL] => N
[USE_GIFTS_MAIN_PR_SECTION_LIST] => N
[GIFTS_SHOW_DISCOUNT_PERCENT] => Y
[GIFTS_SHOW_OLD_PRICE] => Y
[GIFTS_DETAIL_PAGE_ELEMENT_COUNT] => 4
[GIFTS_DETAIL_HIDE_BLOCK_TITLE] => N
[GIFTS_DETAIL_TEXT_LABEL_GIFT] => Подарок
[GIFTS_DETAIL_BLOCK_TITLE] => Выберите один из подарков
[GIFTS_SHOW_NAME] => Y
[GIFTS_SHOW_IMAGE] => Y
[GIFTS_MESS_BTN_BUY] => Выбрать
[GIFTS_PRODUCT_BLOCKS_ORDER] => price,props,sku,quantityLimit,quantity,buttons
[GIFTS_SHOW_SLIDER] => Y
[GIFTS_SLIDER_INTERVAL] => 3000
[GIFTS_SLIDER_PROGRESS] => N
[GIFTS_MAIN_PRODUCT_DETAIL_PAGE_ELEMENT_COUNT] => 4
[GIFTS_MAIN_PRODUCT_DETAIL_BLOCK_TITLE] => Выберите один из товаров, чтобы получить подарок
[GIFTS_MAIN_PRODUCT_DETAIL_HIDE_BLOCK_TITLE] => N
[USER_CONSENT] => N
[USER_CONSENT_ID] => 0
[USER_CONSENT_IS_CHECKED] => Y
[USER_CONSENT_IS_LOADED] => N
[PRODUCT_DISPLAY_MODE] => Y
[CURRENT_BASE_PAGE] => /en/catalog/nabory-i-smesi-ot-otpcr/biomaster-qrt-pcr-2-/
[PARENT_NAME] => bitrix:catalog
[PARENT_TEMPLATE_NAME] => catalog_en
[PARENT_TEMPLATE_PAGE] => element
[CHECK_LANDING_PRODUCT_SECTION] =>
[SHOW_SKU_DESCRIPTION] => N
[HIDE_DETAIL_URL] =>
[ACTION_COMPARE_VARIABLE] => action
[SET_BROWSER_TITLE] => Y
[SET_META_KEYWORDS] => Y
[SET_META_DESCRIPTION] => Y
[COMPARE_NAME] => CATALOG_COMPARE_LIST
[FILL_ITEM_ALL_PRICES] =>
[OFFERS_LIMIT] => 0
[SHOW_FROM_SECTION] => N
[FILTER_IDS] => Array
(
)
[FROM_SECTION] =>
[SECTIONS_CHAIN_START_FROM] => 0
[SHOW_WORKFLOW] =>
[ADDITIONAL_FILTER_NAME] =>
[~IBLOCK_ID] => 10
[~IBLOCK_TYPE] => catalog
[~PROPERTY_CODE] => Array
(
)
[~META_KEYWORDS] => -
[~META_DESCRIPTION] => -
[~BROWSER_TITLE] => NAME
[~SET_CANONICAL_URL] => N
[~BASKET_URL] => /en/cart/
[~ACTION_VARIABLE] => action
[~PRODUCT_ID_VARIABLE] => id
[~SECTION_ID_VARIABLE] => SECTION_ID
[~CHECK_SECTION_ID_VARIABLE] => N
[~PRODUCT_QUANTITY_VARIABLE] => quantity
[~PRODUCT_PROPS_VARIABLE] => prop
[~CACHE_TYPE] => A
[~CACHE_TIME] => 36000000
[~CACHE_GROUPS] => Y
[~SET_TITLE] => 1
[~SET_LAST_MODIFIED] =>
[~MESSAGE_404] =>
[~SET_STATUS_404] => N
[~SHOW_404] => N
[~FILE_404] =>
[~PRICE_CODE] => Array
(
[0] => base_price
)
[~USE_PRICE_COUNT] =>
[~SHOW_PRICE_COUNT] => 1
[~PRICE_VAT_INCLUDE] =>
[~PRICE_VAT_SHOW_VALUE] =>
[~USE_PRODUCT_QUANTITY] => 1
[~PRODUCT_PROPERTIES] => Array
(
)
[~ADD_PROPERTIES_TO_BASKET] => Y
[~PARTIAL_PRODUCT_PROPERTIES] => N
[~LINK_IBLOCK_TYPE] =>
[~LINK_IBLOCK_ID] => 0
[~LINK_PROPERTY_SID] =>
[~LINK_ELEMENTS_URL] => link.php?PARENT_ELEMENT_ID=#ELEMENT_ID#
[~OFFERS_CART_PROPERTIES] => Array
(
)
[~OFFERS_FIELD_CODE] => Array
(
)
[~OFFERS_PROPERTY_CODE] => Array
(
[0] => PREVIEW_PICTURE
[1] => DETAIL_PICTURE
)
[~OFFERS_SORT_FIELD] => sort
[~OFFERS_SORT_ORDER] => asc
[~OFFERS_SORT_FIELD2] => id
[~OFFERS_SORT_ORDER2] => desc
[~ELEMENT_ID] => 0
[~ELEMENT_CODE] => biomaster-qrt-pcr-2-
[~SECTION_ID] => 0
[~SECTION_CODE] => nabory-i-smesi-ot-otpcr
[~SECTION_URL] => /en/catalog/#SECTION_CODE_PATH#/
[~DETAIL_URL] => /en/catalog/#SECTION_CODE_PATH#/#ELEMENT_CODE#/
[~CONVERT_CURRENCY] => N
[~CURRENCY_ID] =>
[~HIDE_NOT_AVAILABLE] => N
[~HIDE_NOT_AVAILABLE_OFFERS] => N
[~USE_ELEMENT_COUNTER] => Y
[~SHOW_DEACTIVATED] => N
[~USE_MAIN_ELEMENT_SECTION] =>
[~STRICT_SECTION_CHECK] =>
[~ADD_PICT_PROP] => -
[~LABEL_PROP] => Array
(
)
[~LABEL_PROP_MOBILE] =>
[~LABEL_PROP_POSITION] =>
[~OFFER_ADD_PICT_PROP] => -
[~OFFER_TREE_PROPS] => Array
(
)
[~PRODUCT_SUBSCRIPTION] => N
[~SHOW_DISCOUNT_PERCENT] => N
[~DISCOUNT_PERCENT_POSITION] =>
[~SHOW_OLD_PRICE] => N
[~SHOW_MAX_QUANTITY] => N
[~MESS_SHOW_MAX_QUANTITY] =>
[~RELATIVE_QUANTITY_FACTOR] =>
[~MESS_RELATIVE_QUANTITY_MANY] =>
[~MESS_RELATIVE_QUANTITY_FEW] =>
[~MESS_BTN_BUY] => Купить
[~MESS_BTN_ADD_TO_BASKET] => В корзину
[~MESS_BTN_SUBSCRIBE] => Подписаться
[~MESS_BTN_DETAIL] => Подробнее
[~MESS_NOT_AVAILABLE] => Нет в наличии
[~MESS_BTN_COMPARE] => Сравнение
[~MESS_PRICE_RANGES_TITLE] => Цены
[~MESS_DESCRIPTION_TAB] => Описание
[~MESS_PROPERTIES_TAB] => Характеристики
[~MESS_COMMENTS_TAB] => Комментарии
[~MAIN_BLOCK_PROPERTY_CODE] => Array
(
[0] => ITEM_ID
[1] => ITEM_PHOTOS
[2] => ITEM_PASSPORT
[3] => ITEM_AD_BUKLET
[4] => ITEM_DOCUMENTATION
[5] => ITEM_SPECIALITY
[6] => ITEM_REACTION_SMES
)
[~MAIN_BLOCK_OFFERS_PROPERTY_CODE] => Array
(
[0] => QUANTITY_ML
[1] => QUANTITY_REAKCIY_25
[2] => QUANTITY_REAKCIY_50
[3] => QUANTITY_REAKCIY_EA
[4] => QUANTITY_MKG
[5] => REACTION
[6] => QUANTITY_PCS
)
[~USE_VOTE_RATING] => N
[~VOTE_DISPLAY_AS_RATING] =>
[~USE_COMMENTS] => N
[~BLOG_USE] =>
[~BLOG_URL] =>
[~BLOG_EMAIL_NOTIFY] =>
[~VK_USE] =>
[~VK_API_ID] => API_ID
[~FB_USE] =>
[~FB_APP_ID] =>
[~BRAND_USE] => N
[~BRAND_PROP_CODE] =>
[~DISPLAY_NAME] => Y
[~IMAGE_RESOLUTION] => 16by9
[~PRODUCT_INFO_BLOCK_ORDER] => sku,props
[~PRODUCT_PAY_BLOCK_ORDER] => rating,price,priceRanges,quantityLimit,quantity,buttons
[~ADD_DETAIL_TO_SLIDER] => N
[~TEMPLATE_THEME] => blue
[~ADD_SECTIONS_CHAIN] => 1
[~ADD_ELEMENT_CHAIN] => 1
[~DISPLAY_PREVIEW_TEXT_MODE] => E
[~DETAIL_PICTURE_MODE] => Array
(
)
[~ADD_TO_BASKET_ACTION] => Array
(
[0] => BUY
)
[~ADD_TO_BASKET_ACTION_PRIMARY] => Array
(
[0] => BUY
)
[~SHOW_CLOSE_POPUP] => N
[~DISPLAY_COMPARE] =>
[~COMPARE_PATH] => /en/catalog/compare.php?action=#ACTION_CODE#
[~USE_COMPARE_LIST] => Y
[~BACKGROUND_IMAGE] =>
[~COMPATIBLE_MODE] => Y
[~DISABLE_INIT_JS_IN_COMPONENT] => N
[~SET_VIEWED_IN_COMPONENT] => N
[~SHOW_SLIDER] => N
[~SLIDER_INTERVAL] =>
[~SLIDER_PROGRESS] =>
[~USE_ENHANCED_ECOMMERCE] => N
[~DATA_LAYER_NAME] =>
[~BRAND_PROPERTY] =>
[~USE_GIFTS_DETAIL] => N
[~USE_GIFTS_MAIN_PR_SECTION_LIST] => N
[~GIFTS_SHOW_DISCOUNT_PERCENT] => Y
[~GIFTS_SHOW_OLD_PRICE] => Y
[~GIFTS_DETAIL_PAGE_ELEMENT_COUNT] => 4
[~GIFTS_DETAIL_HIDE_BLOCK_TITLE] => N
[~GIFTS_DETAIL_TEXT_LABEL_GIFT] => Подарок
[~GIFTS_DETAIL_BLOCK_TITLE] => Выберите один из подарков
[~GIFTS_SHOW_NAME] => Y
[~GIFTS_SHOW_IMAGE] => Y
[~GIFTS_MESS_BTN_BUY] => Выбрать
[~GIFTS_PRODUCT_BLOCKS_ORDER] => price,props,sku,quantityLimit,quantity,buttons
[~GIFTS_SHOW_SLIDER] => Y
[~GIFTS_SLIDER_INTERVAL] => 3000
[~GIFTS_SLIDER_PROGRESS] => N
[~GIFTS_MAIN_PRODUCT_DETAIL_PAGE_ELEMENT_COUNT] => 4
[~GIFTS_MAIN_PRODUCT_DETAIL_BLOCK_TITLE] => Выберите один из товаров, чтобы получить подарок
[~GIFTS_MAIN_PRODUCT_DETAIL_HIDE_BLOCK_TITLE] => N
[~USER_CONSENT] => N
[~USER_CONSENT_ID] => 0
[~USER_CONSENT_IS_CHECKED] => Y
[~USER_CONSENT_IS_LOADED] => N
[~PRODUCT_DISPLAY_MODE] => Y
[~CURRENT_BASE_PAGE] => /en/catalog/nabory-i-smesi-ot-otpcr/biomaster-qrt-pcr-2-/
[~PARENT_NAME] => bitrix:catalog
[~PARENT_TEMPLATE_NAME] => catalog_en
[~PARENT_TEMPLATE_PAGE] => element
[~CHECK_LANDING_PRODUCT_SECTION] =>
[~SHOW_SKU_DESCRIPTION] => N
[~HIDE_DETAIL_URL] =>
[~ACTION_COMPARE_VARIABLE] => action
[~SET_BROWSER_TITLE] => Y
[~SET_META_KEYWORDS] => Y
[~SET_META_DESCRIPTION] => Y
[~COMPARE_NAME] => CATALOG_COMPARE_LIST
[~FILL_ITEM_ALL_PRICES] =>
[~OFFERS_LIMIT] => 0
[~SHOW_FROM_SECTION] => N
[~FILTER_IDS] => Array
(
)
[~FROM_SECTION] =>
[~SECTIONS_CHAIN_START_FROM] => 0
[~SHOW_WORKFLOW] =>
[~ADDITIONAL_FILTER_NAME] =>
)
Application
Gene expression analysis;
One-step RT-PCR.
RM03-80
Number of reactions (25 μl)
80
€57
RM03-400
Number of reactions (25 μl)
400
€250
RM03-400
Number of reactions (25 μl)
400
€2,000
Basic description
BioMaster RT-qPCR (2×) reagent kit includes 2× buffer for RT-qPCR containing all components (except for DNA template and primers), 25× BioMaster-mix and DEPC-treated water. The kit is designed for one-step reverse transcription and real-time polymerase chain reaction (RT-qPCR) with fluorescent probes.
25× BioMaster-mix contains the optimal ratio of M-MuLV –RH to HS-Taq DNA polymerase for both reactions.
M-MuLV –RH is a genetically modified Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV). The enzyme exerts RNA- and DNA-dependent polymerase activity but lacks RNase H activity. M-MuLV –RH reverse transcriptase exhibits improved thermal stability and is active at high temperatures (up to 50°С).
HS-Taq DNA polymerase is a recombinant Taq DNA Polymerase inactivated by specific monoclonal antibodies. The enzyme is inactive at temperatures up to 70 °C, it is activated at the first PCR cycle during a short 5-min incubation at 95 °C. Recombinant HS-Taq DNA polymerase catalyzes 5’→3’ synthesis of DNA and possesses 5’→3’ exonuclease activity of the native Taq DNA polymerase from Thermus aquaticus. Recombinant HS-Taq DNA polymerase is ideal for conventional PCR of the templates of up to 5 kbp.
2× buffer for RT-qPCR is optimized for both efficient RT and PCR.
Product composition
2× buffer for RT-qPCR
25× BioMaster-mix
DEPC-treated water
Number of reactions
RM03-40
2 × 0.5 ml
1 × 80 µl
0.5 ml
2 × 0.5 ml
40
RM03-200
4 × 1.25 ml
2 × 200 µl
0.5 ml
3 × 1.8 ml
200
2× buffer for RT-qPCR contains:
100 mM Tris-НCl (рН 8.3 at 25 °С), 150 mM KCl, 0.6 mM of each deoxynucleoside triphosphate, 6 mM MgCl2 , 10 mM TCEP, enzyme stabilizers and enhancers.
25× BioMaster-mix contains:
50 mM Tris-НCl (рН 8.0 at 25 °С), 100 mM NaCl, 1 mM EDTA, 5 mM DTT, 50 % (v/v) glycerol and 0.1 % (v/v) NP-40, M-MuLV –RH reverse transcriptase, highly-processive HS-Taq DNA polymerase and inhibitor of RNases.
Limits of use
Areas of use
Gene Expression Analysis RT-PCR one- and two-step Reaction mixture properties
The reaction mix is optimized for the specific and effective performance of M-MuLV –RH reverse transcriptase and HS-Taq DNA polymerase;
The mix contains substances that allow long-term storage (storage of BioMaster RT-qPCR (2×) at room temperature for 2 days does not affect RT-PCR efficacy) and multiple thawing-freezing cycles;
Does not contain dyes, which makes the reaction mix multi-purpose.
Benefits of using
Increased specificity
High sensitivity
Easy and convenient in use
Low pipetting error and low risk of cross-contamination
Standardized conditions of the same-type reactions (reduced pipetting error during mixing PCR components in a series of experiments)
PCR products can be further subjected to TA cloning due to deoxyadenosine overhangs at the ends of amplified DNA fragments.
Storage
at +4 ° C – 1 week; at -20 ° С - 1 year; not more than 30 thawing-freezing cycles.
Transportation
at 0 - +4 ° C, it is allowed to transport at room temperature up to 8 days.
Product passport
We carefully prepare our products and are ready to confirm their suitability with a passport.
Each box of our products has a unique lot number in the AA-000-0000 format.
