BioMaster RT-PCR-Extra (2 ×)

Application
  • Gene Expression Analysis
  • One-Step Standard RT-PCR
  • One-step standard RT-PCR using fluorescent probes
  • One-step RT-PCR in real time with SYBR Green I fluorescent dye


Basic description

BioMaster RT-PCR-Extra (2 ×) is designed for reverse transcription and polymerase chain reaction (RT-PCR) with long up to 9 kbp. and complex RNA templates using a one-step method. BioMaster RT-PCR-Extra (2 ×) contains the following optimal RT-PCR: RNAscribe RT reverse transcriptase, HS-Taq DNA polymerase and Pfu DNA polymerase. The advantage of BioMaster RT-PCR-Extra (2 ×) is the possibility of TA cloning of PCR products due to the deoxyadenosine residues protruding at the ends of the amplified DNA paragraphs.

Areas of use

  • Gene Expression Analysis
  • RT-PCR one- and two-step

Benefits of using

  • High specificity
  • High sensitivity
  • Simplicity and ease of use
  • Low pipetting error and cross-contamination
  • The conditions for staging the same type of reactions are standardized (the error decreases when mixing PCR components in different experiments)
  • Possibility of TA cloning of PCR products due to deoxyadenosine residues protruding at the ends of the amplified DNA paragraphs.

Storage

Transportation

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