BioMaster RT-PCR – Premium (2×)

Application
  • Gene Expression Analysis
  • One-Step Standard RT-PCR
  • One-step standard RT-PCR using fluorescent probes
  • One-step RT-PCR in real time with SYBR Green I fluorescent dye

Basic description

BioMaster RT-PCR-Premium and BioMaster RT-PCR-Premium-Color (2 ×) are designed for reverse transcription and polymerase chain reaction (RT-PCR) from long (up to 7 kb) and complex RNA templates by a one-step method followed by detection by gel electrophoresis. The combination of the enzymes M-MuLV –RH, HS-Taq DNA polymerase and Pfu DNA polymerase made it possible to increase the accuracy and reliability of amplification several times compared to Taq DNA polymerase. BioMaster RT-PCR-Premium-Color (2 ×) includes a buffer (6x) for application to the gel as well as a buffer with an increased solution density and an inert marker colouring agent that does not reduce the PCR efficiency and facilitates application to the gel.

Areas of use

  • Gene Expression Analysis
  • RT-PCR one- and two-step

Benefits of using

  • High specificity
  • High sensitivity
  • Simplicity and ease of use
  • Low pipetting error and cross-contamination
  • The conditions for staging the same type of reactions are standardized (the error decreases when mixing PCR components in different experiments)
  • Possibility of TA cloning of PCR products due to deoxyadenosine residues protruding at the ends of the amplified DNA paragraphs.

Storage

Transportation

Product passport

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Each box of our products has a unique lot number in the AA-000-0000 format.
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