BioMaster RT-PCR – Premium (2×)

  • Gene Expression Analysis
  • One-Step Standard RT-PCR
  • One-step standard RT-PCR using fluorescent probes
  • One-step RT-PCR in real time with SYBR Green I fluorescent dye

Basic description

BioMaster RT-PCR-Premium and BioMaster RT-PCR-Premium-Color (2 ×) are designed for reverse transcription and polymerase chain reaction (RT-PCR) from long (up to 7 kb) and complex RNA templates by a one-step method followed by detection by gel electrophoresis. The combination of the enzymes M-MuLV –RH, HS-Taq DNA polymerase and Pfu DNA polymerase made it possible to increase the accuracy and reliability of amplification several times compared to Taq DNA polymerase. BioMaster RT-PCR-Premium-Color (2 ×) includes a buffer (6x) for application to the gel as well as a buffer with an increased solution density and an inert marker colouring agent that does not reduce the PCR efficiency and facilitates application to the gel.

Areas of use

  • Gene Expression Analysis
  • RT-PCR one- and two-step

Benefits of using

  • High specificity
  • High sensitivity
  • Simplicity and ease of use
  • Low pipetting error and cross-contamination
  • The conditions for staging the same type of reactions are standardized (the error decreases when mixing PCR components in different experiments)
  • Possibility of TA cloning of PCR products due to deoxyadenosine residues protruding at the ends of the amplified DNA paragraphs.



Product passport

We carefully prepare our products and are ready to confirm their suitability with a passport.

Each box of our products has a unique lot number in the AA-000-0000 format.
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