Biomaster RT-PCR-Standard (2x)

Application
  • Gene Expression Analysis
  • One-Step Standard RT-PCR
  • One-step standard RT-PCR using fluorescent probes
  • One-step RT-PCR in real time with SYBR Green I fluorescent dye


Basic description

Biomaster RT-PCR-Standard (2x) and BioMaster RT-PCR - Color (2x ) are designed for reverse transcription and polymerase chain reaction (RT-PCR) by a single-step method followed by detection by electrophoresis. The Biomaster RT-PCR-Standard (2x) contains a mix of M-MuLV-RH and HS-Taq DNA polymerase and buffer solution for application (6x) for subsequent detection with gel electrophoresis. And BioMaster RT-PCR - Color (2x ) contains 2 × buffer solutions, its increased density and marker colouring agents make the application to the gel easier.

It is not recommended to use them for amplicons longer than 5 kbp.

Areas of use

  • Gene Expression Analysis
  • RT-PCR one- and two-step

Benefits of using

  • High specificity
  • High sensitivity
  • Simplicity and ease of use
  • Low pipetting error and cross-contamination
  • The conditions for staging the same type of reactions are standardized (the error decreases when mixing PCR components in different experiments)
  • Possibility of TA cloning of PCR products due to deoxyadenosine residues protruding at the ends of the amplified DNA paragraphs.

Storage

Transportation

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