BioMaster LR HS-PCR (2×) includes 2× BioMaster LR HS-PCR (2×) reaction mix, sterile water and 6× loading buffer. The master mix is developed for amplification of long (0.2 through 30 kbp) DNA fragments with enhanced specificity, high fidelity and reaction yield. The mix is also ideally suitable for amplification of GC-rich (>65%) and complicated DNA regions. BioMaster LR HS-PCR (2×) contains all components necessary for PCR (except for DNA template and primers): polymerase mix (HS-Taq and Pfu), deoxynucleoside triphosphate mix, PCR buffer, and Mg2+.
BioMaster LR HS-PCR (2×) mix contains a combination of two highly purified enzymes: highly processive HS-Taq DNA polymerase and Pfu DNA polymerase with correction function. The mixture of polymerases is inactive at room temperature (hot start), and their activation requires preheating of the reaction solution at 95 °C for 5 min.
The combination of polymerases enables to enhance fidelity and reliability of amplification reaction several fold compared to PCR with Taq polymerase only. Synergic performance of the two enzymes allows to obtain PCR products up to 30 kbp. The products obtained using BioMaster LR HS-PCR (2×) mostly contain 3´-dA ends. Hence, they can be further used for cloning.
The buffer is optimized for efficient performance of both polymerases and provides high reaction yield. Use of the kit helps saving time and minimizes contamination risk due to reduced number of pipetting steps.
Areas of use
PCR complex and GC-rich regions
PCR for obtaining long fragments "long-range" PCR
Reaction mixture properties
Optimized for specific performance of Taq and Pfu DNA polymerases;
Composition of the mixture allows long-term storage (the storage of BioMaster LR HS-PCR (2×) at room temperature for 7 days does not affect PCR efficiency) and multiple thawing-freezing cycles (more than 50 times)
Benefits of using
Amplification of long fragments:
up to 30 kbp (viral DNA templates)
up to 15 kbp (genomic DNA templates)
Enhanced amplification fidelity compared to that performed using Taq DNA polymerase only
Hot-start enzyme increases specificity, sensitivity and reaction yield
Activation of HS-Taq DNA polymerase takes not more than 5 min
Amplification of a wide spectrum of DNA templates
PCR products can be further subjected to TA cloning due to the presence of deoxyadenosine overhangs in amplified DNA
1 year (under proper storage and transportation conditions).
at -20 °С; not more than 50 thawing-freezing cycles.
We carefully prepare our products and are ready to confirm their suitability with a passport.
Each box of our products has a unique lot number in the AA-000-0000 format.
Enter it in the box below and download the certificate in PDF format.