BioMaster LR HS-PCR-Color (2×) includes 2× BioMaster LR HS-PCR-Color (2×) mix, DMSO and sterile water. The master mix is developed for amplification of long (0.2 through 30 kbp) DNA fragments with enhanced specificity, high fidelity and reaction yield. The mix is also ideally suitable for amplification of GC-rich (>65%) and complicated DNA regions. BioMaster LR HS-PCR-Color (2×) contains all the components necessary for PCR (except for DNA template and primers): polymerase mix (HS-Taq and Pfu), deoxynucleoside triphosphate mix, PCR buffer, Mg2+, and marker dyes.
BioMaster LR HS-PCR-Color (2×) mix contains a combination of two highly purified enzymes: highly processive HS-Taq DNA polymerase and Pfu DNA polymerase with correction function. The mixture of polymerases is inactive at room temperature (hot start), and their activation requires preheating of the reaction solution at 95 °C for 5 min.
The combination of polymerases enabled to enhance fidelity and reliability of amplification several fold compared to PCR with Taq polymerase only. Synergic performance of the two enzymes allows to obtain PCR products up to 30 kbp in length. The products obtained using BioMaster LR HS-PCR-Color (2×) mostly contain 3´-dA ends. Hence, they can be further used for cloning.
The buffer is optimized for efficient performance of both polymerases and provides high reaction yield. High density of the solution and the presence of marker dyes allow direct gel loading. Use of the kit helps saving time and minimizes contamination risk due to reduced number of pipetting steps. Dyes included in the mix do not interfere with amplicon purification by most of the available methods.