Array
(
[TEMPLATE_THEME] => blue
[SHOW_MAX_QUANTITY] => N
[SHOW_OLD_PRICE] => N
[SHOW_CLOSE_POPUP] => N
[SHOW_DISCOUNT_PERCENT] => N
[DISCOUNT_PERCENT_POSITION] => bottom-right
[LABEL_PROP] => Array
(
)
[LABEL_PROP_MOBILE] => Array
(
)
[LABEL_PROP_POSITION] => top-left
[PRODUCT_SUBSCRIPTION] => N
[MESS_BTN_BUY] => Купить
[MESS_BTN_ADD_TO_BASKET] => В корзину
[MESS_BTN_SUBSCRIBE] => Подписаться
[MESS_BTN_DETAIL] => Подробнее
[MESS_NOT_AVAILABLE] => Нет в наличии
[MESS_BTN_COMPARE] => Сравнение
[SHOW_SLIDER] => N
[SLIDER_INTERVAL] => 5000
[SLIDER_PROGRESS] => N
[USE_ENHANCED_ECOMMERCE] => N
[DATA_LAYER_NAME] =>
[BRAND_PROPERTY] =>
[DISPLAY_NAME] => Y
[USE_RATIO_IN_RANGES] => Y
[IMAGE_RESOLUTION] => 16by9
[DETAIL_PICTURE_MODE] => Array
(
)
[ADD_DETAIL_TO_SLIDER] => N
[DISPLAY_PREVIEW_TEXT_MODE] => E
[ADD_TO_BASKET_ACTION] => Array
(
[0] => BUY
)
[ADD_TO_BASKET_ACTION_PRIMARY] => Array
(
[0] => BUY
)
[USE_VOTE_RATING] => N
[VOTE_DISPLAY_AS_RATING] => rating
[USE_COMMENTS] => N
[BLOG_USE] => N
[BLOG_URL] =>
[BLOG_EMAIL_NOTIFY] =>
[VK_USE] => N
[VK_API_ID] => API_ID
[FB_USE] => N
[FB_APP_ID] =>
[BRAND_USE] => N
[BRAND_PROP_CODE] => Array
(
)
[IBLOCK_ID] => 10
[IBLOCK_TYPE] => catalog
[PROPERTY_CODE] => Array
(
)
[META_KEYWORDS] => -
[META_DESCRIPTION] => -
[BROWSER_TITLE] => NAME
[SET_CANONICAL_URL] => N
[BASKET_URL] => /en/cart/
[ACTION_VARIABLE] => action
[PRODUCT_ID_VARIABLE] => id
[SECTION_ID_VARIABLE] => SECTION_ID
[CHECK_SECTION_ID_VARIABLE] => N
[PRODUCT_QUANTITY_VARIABLE] => quantity
[PRODUCT_PROPS_VARIABLE] => prop
[CACHE_TYPE] => A
[CACHE_TIME] => 36000000
[CACHE_GROUPS] => Y
[SET_TITLE] => 1
[SET_LAST_MODIFIED] =>
[MESSAGE_404] =>
[SET_STATUS_404] => N
[SHOW_404] => N
[FILE_404] =>
[PRICE_CODE] => Array
(
[0] => base_price
)
[USE_PRICE_COUNT] =>
[SHOW_PRICE_COUNT] => 1
[PRICE_VAT_INCLUDE] =>
[PRICE_VAT_SHOW_VALUE] =>
[USE_PRODUCT_QUANTITY] => 1
[PRODUCT_PROPERTIES] => Array
(
)
[ADD_PROPERTIES_TO_BASKET] => Y
[PARTIAL_PRODUCT_PROPERTIES] => N
[LINK_IBLOCK_TYPE] =>
[LINK_IBLOCK_ID] => 0
[LINK_PROPERTY_SID] =>
[LINK_ELEMENTS_URL] => link.php?PARENT_ELEMENT_ID=#ELEMENT_ID#
[OFFERS_CART_PROPERTIES] => Array
(
)
[OFFERS_FIELD_CODE] => Array
(
)
[OFFERS_PROPERTY_CODE] => Array
(
[0] => PREVIEW_PICTURE
[1] => DETAIL_PICTURE
)
[OFFERS_SORT_FIELD] => sort
[OFFERS_SORT_ORDER] => asc
[OFFERS_SORT_FIELD2] => id
[OFFERS_SORT_ORDER2] => desc
[ELEMENT_ID] => 811
[ELEMENT_CODE] => biomaster-lr-hs-pcr-color-2x
[SECTION_ID] => 0
[SECTION_CODE] => prc-long-fragments
[SECTION_URL] => /en/catalog/#SECTION_CODE_PATH#/
[DETAIL_URL] => /en/catalog/#SECTION_CODE_PATH#/#ELEMENT_CODE#/
[CONVERT_CURRENCY] => N
[CURRENCY_ID] =>
[HIDE_NOT_AVAILABLE] => N
[HIDE_NOT_AVAILABLE_OFFERS] => N
[USE_ELEMENT_COUNTER] => Y
[SHOW_DEACTIVATED] => N
[USE_MAIN_ELEMENT_SECTION] =>
[STRICT_SECTION_CHECK] =>
[ADD_PICT_PROP] =>
[OFFER_ADD_PICT_PROP] =>
[OFFER_TREE_PROPS] => Array
(
)
[MESS_SHOW_MAX_QUANTITY] => Stock
[RELATIVE_QUANTITY_FACTOR] => 5
[MESS_RELATIVE_QUANTITY_MANY] => in stock
[MESS_RELATIVE_QUANTITY_FEW] => only a few left
[MESS_PRICE_RANGES_TITLE] => Цены
[MESS_DESCRIPTION_TAB] => Описание
[MESS_PROPERTIES_TAB] => Характеристики
[MESS_COMMENTS_TAB] => Комментарии
[MAIN_BLOCK_PROPERTY_CODE] => Array
(
[ITEM_ID] => 0
[ITEM_PHOTOS] => 1
[ITEM_PASSPORT] => 2
[ITEM_AD_BUKLET] => 3
[ITEM_DOCUMENTATION] => 4
[ITEM_SPECIALITY] => 5
[ITEM_REACTION_SMES] => 6
)
[MAIN_BLOCK_OFFERS_PROPERTY_CODE] => Array
(
[QUANTITY_ML] => 0
[QUANTITY_REAKCIY_25] => 1
[QUANTITY_REAKCIY_50] => 2
[QUANTITY_REAKCIY_EA] => 3
[QUANTITY_MKG] => 4
[REACTION] => 5
[QUANTITY_PCS] => 6
)
[PRODUCT_INFO_BLOCK_ORDER] => Array
(
[0] => sku
[1] => props
)
[PRODUCT_PAY_BLOCK_ORDER] => Array
(
[0] => rating
[1] => price
[2] => priceRanges
[3] => quantityLimit
[4] => quantity
[5] => buttons
)
[ADD_SECTIONS_CHAIN] => 1
[ADD_ELEMENT_CHAIN] => 1
[DISPLAY_COMPARE] =>
[COMPARE_PATH] => /en/catalog/compare.php?action=#ACTION_CODE#
[USE_COMPARE_LIST] => Y
[BACKGROUND_IMAGE] =>
[COMPATIBLE_MODE] => Y
[DISABLE_INIT_JS_IN_COMPONENT] => N
[SET_VIEWED_IN_COMPONENT] => N
[USE_GIFTS_DETAIL] => N
[USE_GIFTS_MAIN_PR_SECTION_LIST] => N
[GIFTS_SHOW_DISCOUNT_PERCENT] => Y
[GIFTS_SHOW_OLD_PRICE] => Y
[GIFTS_DETAIL_PAGE_ELEMENT_COUNT] => 4
[GIFTS_DETAIL_HIDE_BLOCK_TITLE] => N
[GIFTS_DETAIL_TEXT_LABEL_GIFT] => Подарок
[GIFTS_DETAIL_BLOCK_TITLE] => Выберите один из подарков
[GIFTS_SHOW_NAME] => Y
[GIFTS_SHOW_IMAGE] => Y
[GIFTS_MESS_BTN_BUY] => Выбрать
[GIFTS_PRODUCT_BLOCKS_ORDER] => price,props,sku,quantityLimit,quantity,buttons
[GIFTS_SHOW_SLIDER] => Y
[GIFTS_SLIDER_INTERVAL] => 3000
[GIFTS_SLIDER_PROGRESS] => N
[GIFTS_MAIN_PRODUCT_DETAIL_PAGE_ELEMENT_COUNT] => 4
[GIFTS_MAIN_PRODUCT_DETAIL_BLOCK_TITLE] => Выберите один из товаров, чтобы получить подарок
[GIFTS_MAIN_PRODUCT_DETAIL_HIDE_BLOCK_TITLE] => N
[USER_CONSENT] => N
[USER_CONSENT_ID] => 0
[USER_CONSENT_IS_CHECKED] => Y
[USER_CONSENT_IS_LOADED] => N
[PRODUCT_DISPLAY_MODE] => Y
[CURRENT_BASE_PAGE] => /en/catalog/nabory-i-smesi-prc/prc-long-fragments/biomaster-lr-hs-pcr-color-2x/
[PARENT_NAME] => bitrix:catalog
[PARENT_TEMPLATE_NAME] => catalog_en
[PARENT_TEMPLATE_PAGE] => element
[CHECK_LANDING_PRODUCT_SECTION] =>
[HIDE_DETAIL_URL] =>
[ACTION_COMPARE_VARIABLE] => action
[SET_BROWSER_TITLE] => Y
[SET_META_KEYWORDS] => Y
[SET_META_DESCRIPTION] => Y
[COMPARE_NAME] => CATALOG_COMPARE_LIST
[FILL_ITEM_ALL_PRICES] =>
[OFFERS_LIMIT] => 0
[SHOW_FROM_SECTION] => N
[FILTER_IDS] => Array
(
)
[FROM_SECTION] =>
[SECTIONS_CHAIN_START_FROM] => 0
[SHOW_WORKFLOW] =>
[~IBLOCK_ID] => 10
[~IBLOCK_TYPE] => catalog
[~PROPERTY_CODE] => Array
(
)
[~META_KEYWORDS] => -
[~META_DESCRIPTION] => -
[~BROWSER_TITLE] => NAME
[~SET_CANONICAL_URL] => N
[~BASKET_URL] => /en/cart/
[~ACTION_VARIABLE] => action
[~PRODUCT_ID_VARIABLE] => id
[~SECTION_ID_VARIABLE] => SECTION_ID
[~CHECK_SECTION_ID_VARIABLE] => N
[~PRODUCT_QUANTITY_VARIABLE] => quantity
[~PRODUCT_PROPS_VARIABLE] => prop
[~CACHE_TYPE] => A
[~CACHE_TIME] => 36000000
[~CACHE_GROUPS] => Y
[~SET_TITLE] => 1
[~SET_LAST_MODIFIED] =>
[~MESSAGE_404] =>
[~SET_STATUS_404] => N
[~SHOW_404] => N
[~FILE_404] =>
[~PRICE_CODE] => Array
(
[0] => base_price
)
[~USE_PRICE_COUNT] =>
[~SHOW_PRICE_COUNT] => 1
[~PRICE_VAT_INCLUDE] =>
[~PRICE_VAT_SHOW_VALUE] =>
[~USE_PRODUCT_QUANTITY] => 1
[~PRODUCT_PROPERTIES] => Array
(
)
[~ADD_PROPERTIES_TO_BASKET] => Y
[~PARTIAL_PRODUCT_PROPERTIES] => N
[~LINK_IBLOCK_TYPE] =>
[~LINK_IBLOCK_ID] => 0
[~LINK_PROPERTY_SID] =>
[~LINK_ELEMENTS_URL] => link.php?PARENT_ELEMENT_ID=#ELEMENT_ID#
[~OFFERS_CART_PROPERTIES] => Array
(
)
[~OFFERS_FIELD_CODE] => Array
(
)
[~OFFERS_PROPERTY_CODE] => Array
(
[0] => PREVIEW_PICTURE
[1] => DETAIL_PICTURE
)
[~OFFERS_SORT_FIELD] => sort
[~OFFERS_SORT_ORDER] => asc
[~OFFERS_SORT_FIELD2] => id
[~OFFERS_SORT_ORDER2] => desc
[~ELEMENT_ID] => 0
[~ELEMENT_CODE] => biomaster-lr-hs-pcr-color-2x
[~SECTION_ID] => 0
[~SECTION_CODE] => prc-long-fragments
[~SECTION_URL] => /en/catalog/#SECTION_CODE_PATH#/
[~DETAIL_URL] => /en/catalog/#SECTION_CODE_PATH#/#ELEMENT_CODE#/
[~CONVERT_CURRENCY] => N
[~CURRENCY_ID] =>
[~HIDE_NOT_AVAILABLE] => N
[~HIDE_NOT_AVAILABLE_OFFERS] => N
[~USE_ELEMENT_COUNTER] => Y
[~SHOW_DEACTIVATED] => N
[~USE_MAIN_ELEMENT_SECTION] =>
[~STRICT_SECTION_CHECK] =>
[~ADD_PICT_PROP] => -
[~LABEL_PROP] => Array
(
)
[~LABEL_PROP_MOBILE] =>
[~LABEL_PROP_POSITION] =>
[~OFFER_ADD_PICT_PROP] => -
[~OFFER_TREE_PROPS] => Array
(
)
[~PRODUCT_SUBSCRIPTION] => N
[~SHOW_DISCOUNT_PERCENT] => N
[~DISCOUNT_PERCENT_POSITION] =>
[~SHOW_OLD_PRICE] => N
[~SHOW_MAX_QUANTITY] => N
[~MESS_SHOW_MAX_QUANTITY] =>
[~RELATIVE_QUANTITY_FACTOR] =>
[~MESS_RELATIVE_QUANTITY_MANY] =>
[~MESS_RELATIVE_QUANTITY_FEW] =>
[~MESS_BTN_BUY] => Купить
[~MESS_BTN_ADD_TO_BASKET] => В корзину
[~MESS_BTN_SUBSCRIBE] => Подписаться
[~MESS_BTN_DETAIL] => Подробнее
[~MESS_NOT_AVAILABLE] => Нет в наличии
[~MESS_BTN_COMPARE] => Сравнение
[~MESS_PRICE_RANGES_TITLE] => Цены
[~MESS_DESCRIPTION_TAB] => Описание
[~MESS_PROPERTIES_TAB] => Характеристики
[~MESS_COMMENTS_TAB] => Комментарии
[~MAIN_BLOCK_PROPERTY_CODE] => Array
(
[0] => ITEM_ID
[1] => ITEM_PHOTOS
[2] => ITEM_PASSPORT
[3] => ITEM_AD_BUKLET
[4] => ITEM_DOCUMENTATION
[5] => ITEM_SPECIALITY
[6] => ITEM_REACTION_SMES
)
[~MAIN_BLOCK_OFFERS_PROPERTY_CODE] => Array
(
[0] => QUANTITY_ML
[1] => QUANTITY_REAKCIY_25
[2] => QUANTITY_REAKCIY_50
[3] => QUANTITY_REAKCIY_EA
[4] => QUANTITY_MKG
[5] => REACTION
[6] => QUANTITY_PCS
)
[~USE_VOTE_RATING] => N
[~VOTE_DISPLAY_AS_RATING] =>
[~USE_COMMENTS] => N
[~BLOG_USE] =>
[~BLOG_URL] =>
[~BLOG_EMAIL_NOTIFY] =>
[~VK_USE] =>
[~VK_API_ID] => API_ID
[~FB_USE] =>
[~FB_APP_ID] =>
[~BRAND_USE] => N
[~BRAND_PROP_CODE] =>
[~DISPLAY_NAME] => Y
[~IMAGE_RESOLUTION] => 16by9
[~PRODUCT_INFO_BLOCK_ORDER] => sku,props
[~PRODUCT_PAY_BLOCK_ORDER] => rating,price,priceRanges,quantityLimit,quantity,buttons
[~ADD_DETAIL_TO_SLIDER] => N
[~TEMPLATE_THEME] => blue
[~ADD_SECTIONS_CHAIN] => 1
[~ADD_ELEMENT_CHAIN] => 1
[~DISPLAY_PREVIEW_TEXT_MODE] => E
[~DETAIL_PICTURE_MODE] => Array
(
)
[~ADD_TO_BASKET_ACTION] => Array
(
[0] => BUY
)
[~ADD_TO_BASKET_ACTION_PRIMARY] => Array
(
[0] => BUY
)
[~SHOW_CLOSE_POPUP] => N
[~DISPLAY_COMPARE] =>
[~COMPARE_PATH] => /en/catalog/compare.php?action=#ACTION_CODE#
[~USE_COMPARE_LIST] => Y
[~BACKGROUND_IMAGE] =>
[~COMPATIBLE_MODE] => Y
[~DISABLE_INIT_JS_IN_COMPONENT] => N
[~SET_VIEWED_IN_COMPONENT] => N
[~SHOW_SLIDER] => N
[~SLIDER_INTERVAL] =>
[~SLIDER_PROGRESS] =>
[~USE_ENHANCED_ECOMMERCE] => N
[~DATA_LAYER_NAME] =>
[~BRAND_PROPERTY] =>
[~USE_GIFTS_DETAIL] => N
[~USE_GIFTS_MAIN_PR_SECTION_LIST] => N
[~GIFTS_SHOW_DISCOUNT_PERCENT] => Y
[~GIFTS_SHOW_OLD_PRICE] => Y
[~GIFTS_DETAIL_PAGE_ELEMENT_COUNT] => 4
[~GIFTS_DETAIL_HIDE_BLOCK_TITLE] => N
[~GIFTS_DETAIL_TEXT_LABEL_GIFT] => Подарок
[~GIFTS_DETAIL_BLOCK_TITLE] => Выберите один из подарков
[~GIFTS_SHOW_NAME] => Y
[~GIFTS_SHOW_IMAGE] => Y
[~GIFTS_MESS_BTN_BUY] => Выбрать
[~GIFTS_PRODUCT_BLOCKS_ORDER] => price,props,sku,quantityLimit,quantity,buttons
[~GIFTS_SHOW_SLIDER] => Y
[~GIFTS_SLIDER_INTERVAL] => 3000
[~GIFTS_SLIDER_PROGRESS] => N
[~GIFTS_MAIN_PRODUCT_DETAIL_PAGE_ELEMENT_COUNT] => 4
[~GIFTS_MAIN_PRODUCT_DETAIL_BLOCK_TITLE] => Выберите один из товаров, чтобы получить подарок
[~GIFTS_MAIN_PRODUCT_DETAIL_HIDE_BLOCK_TITLE] => N
[~USER_CONSENT] => N
[~USER_CONSENT_ID] => 0
[~USER_CONSENT_IS_CHECKED] => Y
[~USER_CONSENT_IS_LOADED] => N
[~PRODUCT_DISPLAY_MODE] => Y
[~CURRENT_BASE_PAGE] => /en/catalog/nabory-i-smesi-prc/prc-long-fragments/biomaster-lr-hs-pcr-color-2x/
[~PARENT_NAME] => bitrix:catalog
[~PARENT_TEMPLATE_NAME] => catalog_en
[~PARENT_TEMPLATE_PAGE] => element
[~CHECK_LANDING_PRODUCT_SECTION] =>
[~HIDE_DETAIL_URL] =>
[~ACTION_COMPARE_VARIABLE] => action
[~SET_BROWSER_TITLE] => Y
[~SET_META_KEYWORDS] => Y
[~SET_META_DESCRIPTION] => Y
[~COMPARE_NAME] => CATALOG_COMPARE_LIST
[~FILL_ITEM_ALL_PRICES] =>
[~OFFERS_LIMIT] => 0
[~SHOW_FROM_SECTION] => N
[~FILTER_IDS] => Array
(
)
[~FROM_SECTION] =>
[~SECTIONS_CHAIN_START_FROM] => 0
[~SHOW_WORKFLOW] =>
)
Features
2× reaction mix is developed for hot-start amplification of long
DNA fragments ranging from 0.2 to 30 kbp, GC-rich (>65%) and
complicated regions of DNA; contains dyes for easy pipetting and direct
gel loading.
Application
Long-range PCR
Product generation for TA-cloning
Amplification of GC-rich and complicated templates
BioMaster LR HS-PCR-Color (2×) includes 2× BioMaster LR HS-PCR-Color (2×) mix, DMSO and sterile water. The master mix is developed for amplification of long (0.2 through 30 kbp) DNA fragments with enhanced specificity, high fidelity and reaction yield. The mix is also ideally suitable for amplification of GC-rich (>65%) and complicated DNA regions. BioMaster LR HS-PCR-Color (2×) contains all the components necessary for PCR (except for DNA template and primers): polymerase mix (HS-Taq and Pfu), deoxynucleoside triphosphate mix, PCR buffer, Mg2+, and marker dyes.
BioMaster LR HS-PCR-Color (2×) mix contains a combination of two highly purified enzymes: highly processive HS-Taq DNA polymerase and Pfu DNA polymerase with correction function. The mixture of polymerases is inactive at room temperature (hot start), and their activation requires preheating of the reaction solution at 95 °C for 5 min.
The combination of polymerases enabled to enhance fidelity and reliability of amplification several fold compared to PCR with Taq polymerase only. Synergic performance of the two enzymes allows to obtain PCR products up to 30 kbp in length. The products obtained using BioMaster LR HS-PCR-Color (2×) mostly contain 3´-dA ends. Hence, they can be further used for cloning.
The buffer is optimized for efficient performance of both polymerases and provides high reaction yield. High density of the solution and the presence of marker dyes allow direct gel loading. Use of the kit helps saving time and minimizes contamination risk due to reduced number of pipetting steps. Dyes included in the mix do not interfere with amplicon purification by most of the available methods.
Areas of use
PCR for obtaining long fragments "long-range" PCR
Reaction mixture properties
Optimized for specific performance of Taq and Pfu DNA polymerases;
Contains substances that allow long-term storage (the storage of BioMaster LR HS-PCR-Color (2×) at room temperature for 7 days and multiple thawing-freezing cycles (more than 50 times) do not affect PCR efficiency)
Contains dyes that do not affect polymerase performance and components increasing sample density for easy gel loading
Benefits of using
Amplification of long fragments:
up to 30 kbp (viral DNA templates)
up to 15 kbp (genomic DNA templates)
Enhanced amplification fidelity compared to that performed using Taq DNA polymerase only
Hot-start enzyme increases specificity, sensitivity and reaction yield
Activation of HS-Taq DNA polymerase takes not more than 5 min
Amplification of a wide spectrum of DNA templates
Easier gel loading; no need in loading buffer due to high density of the mixture
PCR products can be further subjected to TA cloning due to the presence of deoxyadenosine overhangs in amplified DNA
Storage
1 year (under proper storage and transportation conditions).
Transportation
at -20 °С; not more than 50 thawing-freezing cycles.
Product passport
We carefully prepare our products and are ready to confirm their suitability with a passport.
Each box of our products has a unique lot number in the AA-000-0000 format.
Enter it in the box below and download the certificate in PDF format.
