Реагент «ЛИРА»/Набор «ЛИРА+» для выделения РНК, ДНК и белков
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Особенности
Продукт предназначен для гуанидин тиоцианат-фенол-хлороформной экстракции РНК, ДНК и белков. Реагент разрушает клетки и их компоненты, сохраняя целостность РНК и ДНК. Аналог TRIzol reagent.
Реагент «Лира» предназначен для выделения РНК, ДНК и белков из различных биологических образцов (эукариотических и бактериальных клеток, тканей животных и растений). Продукт представляет собой прозрачный раствор, содержащий фенол и гуанидин тиоцианат. Реагент лизирует образец до гомогенной смеси, которая после смешивания с хлороформом разделяется на нижнюю органическую фазу, интерфазу и верхнюю водную фазу. РНК легко осаждается из водной фазы при помощи изопропанола. Осаждение ДНК происходит из органической фазы и интерфазы при помощи этанола. Белки осаждаются из фенол-этанольного супернатанта изопропанолом.
Важно!
Отличие набора «Лира+» (кат. № LRP-100-2, LRP-100-3) от реагента «Лира» (кат. № LR-100, LR-200) заключается в расширенной комплектации набора «Лира+», включающего, помимо 100 мл реагента «Лира», большинство необходимых реагентов для выделения РНК, ДНК и белков. Раствор для осаждения РНК позволяет выделять РНК из менее чем 10.000 клеток.
Набор «Лира+» для выделения РНК и ДНК LRP-100-2:
Реагент «ЛИРА» 100 мл
PBS 10 мл
Ацетат натрия, 3 М раствор 10 мл
Раствор для осаждения РНК** 1.5 мл
Вода, очищенная от РНКаз* 10 мл
Набор «Лира+» для выделения РНК, ДНК и белков LRP-100-3:
Реагент «ЛИРА» 100 мл
PBS 10 млАцетат натрия, 3 М раствор 10 мл
Раствор для осаждения РНК** 1.5 мл
Вода, очищенная от РНКаз* 10 мл 1 М цитрат натрия (концентрат) 45 мл
2 М NaOH (концентрат) 1.5 мл + + 1 М HEPES 5 мл
Гидрохлорид гуанидина (сухой) 12x1.5г
Додецилсульфат натрия (ДСН) (сухой) 2x0.5 г
*Вода предназначена для растворения выделенной РНК. Вода для приготовления буферов в набор не включена.
**Если в растворе для осаждения РНК при хранении при +4 ⁰C образовался осадок, инкубировать раствор при 30-40 ⁰С до растворения осадок.
Области применения
Выделение РНК, ДНК и белков
Преимущества использования
Хранение
Транспортировка
курьером в регионы РФ на сухом льду, сумма 1200 руб. добавляется к заказу.
Паспорт на продукцию
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На каждой коробке нашей продукции есть уникальный номер-лот в формате АА-000-0000.
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