Electrophoresis Buffers
Ethidium bromide solution for visualization of nucleic acids after agarose gel electrophoresis. The solution was prepared using Type I water and filtered through a 0.22 μm pore size membrane.
10× Buffer for nucleic acid electrophoresis in agarose gel. The buffer was prepared using Type I water and filtered through a 0.45 μm pore size membrane.
Solution for staining and visualization of proteins in polyacrylamide gels after protein electrophoresis according to Laemmli. The solution contains phosphoric acid.
4x Laemmli Protein Electrophoresis Loading Buffer (reducing, with mercaptoethanol) is used to visualize samples when they are applied to a polyacrylamide gel.
4x Laemmli Protein Electrophoresis (Non-Reducing) Loading Buffer is used to visualize samples when they are applied to a polyacrylamide gel.