High-fidelity amplification
High-fidelity DNA polymerases provide exceptional accuracy, speed and performance for all PCR applications.

These enzymes are significantly superior to Pfu and Taq polymerases in the following parameters:
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> processivity
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> synthesis accuracy
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> fragment amplification rate
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> resistance to PCR inhibitors
Comparison of synthesis of long fragments:
Analysis of salt resistance of Pfu DNA polymerase and Fusion DNA polymerase using the example of fragment amplification in the presence of KCl:

Name
Catalog #
Qty
Name
Fusion 2.0 polymerase
A modified version of Fusion DNA polymerase obtained by merging the thermostable DNA polymerase of Pyrococcus furiosus (Pfu) and the DNA-binding protein of the thermophilic archaea species Saccharolobus solfataricus (Sso7d). A number of mutations were added to Fusion 2.0 polymerase, increasing the accuracy of the enzyme by approximately 3 times or ~15 times relative to the “native” Taq DNA polymerase
Name
PCR amplification kit with Fusion DNA polymerase
A set of reagents for performing PCR with high-fidelity DNA polymerase. The kit contains individual components such as magnesium ions, a mixture of deoxynucleotide triphosphates (dNTPs) and dimethyl sulfoxide, which allows you to optimize the amplification conditions for the experimenter’s tasks.
Name
Fusion DNA polymerase (Pfu-Sso7d)
Fusion DNA polymerase consists of Pyrococcus furiosus (Pfu) thermostable DNA polymerase and thermophilic archaea DNA-binding protein Sulfolobus solfataricus (Sso7d). The Sso7d protein binds to the minor groove of double-stranded DNA and stabilizes the polymerase-template complex. Due to this, Fusion DNA polymerase has increased processivity, synthesis accuracy, amplification rate, and resistance to PCR inhibitors.
Name
BioMaster Hi-Fi PCR (2×)
BioMaster Hi-Fi PCR Kit (2×) contains BioMaster Hi-Fi PCR 2× reaction mix, sterile water, and 6× gel loading buffer. The reaction mix is designed for amplification of DNA fragments from 0.1 to 15 kb with high fidelity, enhanced specificity and yield. The reaction mix includes all necessary components for PCR (except DNA template and primers): High-fidelity DNA polymerase (Fusion 2.0),
deoxynucleoside triphosphate mix, PCR buffer, Mg²⁺
Name
PCR amplification kit with Fusion 2.0 DNA polymerase
A reagent kit for performing PCR using high-fidelity Fusion 2.0 polymerase. The kit includes individual components such as reaction buffer, magnesium ions, deox-ynucleotide triphosphate (dNTP) mixture, Fusion 2.0 polymerase, nuclease-free water, and PCR Enhancer, enabling optimization of amplification conditions for the researcher's specific needs.