Enzymes for in vitro transcription
Name
Catalog #
Qty
Name
T7 RNA Polymerase
T7 RNA Polymerase is highly processive DNA-dependent RNA polymerase bacteriophage T7 (T7 RNA polymerase, phage T7 RNA polymerase) that specifically interacts with the T7 promoter and catalyzes the synthesis of RNA fragments in the 5'->3' direction on the DNA template.
Name
RNase inhibitor
The RNase inhibitor is a 50 kDa recombinant protein expressed in E. coli. It inhibits the ribonuclease activity of eukaryotic enzymes such as RNase A, RNase B, RNase C and protects RNA from non-specific hydrolysis.
The RNase inhibitor is intended for use in applications where the presence of RNases can reduce the quality of experimental results, such as RNA isolation, cDNA synthesis, RT-PCR, transcription and translation in vitro.
The RNase inhibitor is intended for use in applications where the presence of RNases can reduce the quality of experimental results, such as RNA isolation, cDNA synthesis, RT-PCR, transcription and translation in vitro.
Name
Inorganic pyrophosphatase
The present product is a recombinant enzyme - inorganic pyrophosphatase of Thermococcus litoralis. The enzyme has a molecular mass of ~21 kDa and catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. The enzyme is active in a wide temperature range and is thermostable.
Name
Thermolabile alkaline phosphatase
This product is a recombinant enzyme - alkaline phosphatase of the gram-negative bacterium Vibrio splendidus.
Application
• Cloning of restriction fragments.
• mRNA synthesis.
Name
DNase (Heat-labile)
The enzyme exhibits high specific activity against double-stranded DNA, while single-stranded DNA or RNA remain intact under standard conditions.