The enzyme exhibits high specific activity against double-stranded DNA, while single-stranded DNA or RNA remain intact under standard conditions.

The RNase inhibitor is a 50 kDa recombinant protein expressed in E. coli. It inhibits the ribonuclease activity of eukaryotic enzymes such as RNase A, RNase B, RNase C and protects RNA from non-specific hydrolysis.
The RNase inhibitor is intended for use in applications where the presence of RNases can reduce the quality of experimental results, such as RNA isolation, cDNA synthesis, RT-PCR, transcription and translation in vitro.

T7 RNA Polymerase is highly processive DNA-dependent RNA polymerase bacteriophage T7 (T7 RNA polymerase, phage T7 RNA polymerase) that specifically interacts with the T7 promoter and catalyzes the synthesis of RNA fragments in the 5'->3' direction on the DNA template.

The principle of operation of the kit is based on the enzymatic synthesis of RNA molecules on a DNA template using DNA-dependent RNA polymerase of bacteriophage T7. The kit includes all the necessary reagents to obtain a high yield of RNA transcripts in a minimum reaction time.

The resulting RNA can be used to study the structure and functions of RNA, for genomic editing systems as a guide RNA, to study the mechanisms of RNA interference, for transfection of cells, for translation in vitro etc.