PCR kits
PCR kits are indispensable reagents in molecular biology laboratories used for nucleic acid amplification.
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Carefully calibrated proportions of the kit allow the user to significantly save time on the experiment, as well as provide a guarantee in obtaining a more accurate reproducible result of the reaction.
PCR kits contain all the necessary components for PCR of different types: kits for classical PCR, kits for PCR with fluorescent dye SYBR Green I, kits for PCR with fluorescent probes, kits for PCR of long fragments (Long-Range), as well as kits for reverse transcription and OT-PCR (reverse transcription PCR).
The main advantages of working with PCR kits are:
- Convenience of their use and availability of all the components needed for PCR assembled in one package.
- Reduction of experiment preparation time.
- Standardization of the initial conditions of the experiment and confidence in the quality of the starting components.
- Reduced likelihood of contamination.
- Simplicity in experiment preparation, availability of detailed instructions.
Basic composition of a PCR kit:
DNA polymerase - usually Taq polymerase, an enzyme originally isolated from the bacterium Thermus aquaticus. This thermostable enzyme can withstand the high temperatures required to denature DNA.
Nucleotides (dNTPs) are the building blocks that Taq polymerase uses to synthesize new DNA strands during PCR. Typically, a mixture of deoxynucleoside triphosphates is balanced from dATP, dGTP, dCTP, dTTP, and dUTP.
The reaction buffer includes magnesium ions as a cofactor for Taq polymerase and provides a suitable chemical environment for PCR.
Primers are short single-stranded oligonucleotides that bind to a target DNA strand and initiate synthesis of the fragment by the polymerase. Kits may include generic primers or primers for a specific gene.
Sterile water - used to replenish the final reaction volume.
Electrophoresis application buffer - if no dye is contained in the mixture. Kits may include dimethyl sulfoxide (DMSO) to increase amplification specificity.
PCR reagent kits vary in composition and concentration of components, allowing you to choose the optimal kit for a particular type of reaction.
In addition, we offer PCR reagent kits that contain special components such as enzymes to improve the specificity and sensitivity of the reaction, intercalating dyes and fluorescent probes, and components to reduce the effects of inhibitors that may be present in the samples.
When selecting a PCR reagent kit, it is important to consider the number of samples, the type of samples to be analyzed, and the specific type of reaction to be performed.
The components of PCR kits are thoroughly tested for performance, and you will end up with consistent reproducible results.
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