BioMaster RT-PCR-Extra (2×) intended for reverse transcription and polymerase chain reaction (RT-PCR) from long ( up to 9 kb) and complex matrices using a one-step method. The kit contains 2× buffer for RT-PCR-Extra containing all necessary components (except for enzymes, RNA template and primers); enzyme mixture BioMaster Extra-Mix, water treated with DEPA, DMSOand application buffer (6x).